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Identification and DNA Fingerprinting of Some Superior Persian Walnut Genotypes in Iran

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Davoodi, Fatemeh ; Rezaei, Mehdi ; Heidari, Parviz ; Hokmabadi, Hossien ; Lawson, Shaneka

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Persian walnut (Juglans regia L.) is a valuable economic plant used worldwide for its wood and nut fruit. In the current study, the genetic variation of 21 selected walnut genotypes from different regions of Iran was described using nut pomological properties, ISSR primers, and DNA analyses based on SSR primers. Nut pomology revealed nut weight of selected genotypes ranged from 12.1 to 17.5g (OR23). Kernel weight varied from 5.3 to 10.3g (OR23), and kernel fill percentage ranged from 43.4 to 62.7% (T12). PCR amplification with ISSR primers revealed 112 bands obtained with 91% polymorphism. Numbers of amplified bands ranged from 9 to 14. The highest primer resolving power (Rp), 7.71, was obtained with the UBC.884 primer and highlights the ability of the most informative primers to differentiate between the genotypes. The average band informativeness (AvIb) varied from 0.14 (UBC.886) to 0.62 (UBC.887 and UBC.888). Using the Jaccard coefficient, genetic similarity values among walnut genotypes varied from 0.52 to 0.88. The dendrogram of UPGMA analysis with ISSR primers classified walnuts genotypes in two main groups and three sub-groups. PCoA largely confirmed cluster analysis results. Genotypes collected from Shahrood displayed lower genetic variation and the Tuyserkan and Urmia genotypes were more genetically distant. Unique DNA profiles were established for all studied genotypes based on three distinct microsatellite loci.


Cluster analysis; Genetic diversity; ISSR marker; SSR marker; Walnut


Davoodi, Fatemeh; Rezaei, Mehdi; Heidari, Parviz; Hokmabadi, Hossien; Lawson, Shaneka. 2021. Identification and DNA Fingerprinting of Some Superior Persian Walnut Genotypes in Iran. Erwerbs-Obstbau. 63(4): 393-402.

Last updated on: April 29, 2022