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Polyamine- and Amino Acid-Related Metabolism: The Roles of Arginine and Ornithine are Associated with the Embryogenic Potential

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de Oliveira, Leandro Francisco; Navarro, Bruno Viana; Cerruti, Giovanni Victório; Elbl, Paula ; Minocha, Rakesh ; Minocha, Subhash C.; dos Santos, André Luis Wendt; Floh, Eny Iochevet Segal.

Year Published

2018

Publication

Plant and Cell Physiology

Abstract

The mechanisms that control polyamine (PA) metabolism in plant cell lines with different embryogenic potential are not well understood. This study involved the use of two Araucaria angustifolia cell lines, one of which was defined as being blocked, in that the cells were incapable of developing somatic embryos, and the other as being responsive, as the cells could generate somatic embryos. Cellular PA metabolism was modulated by using 5mM arginine (Arg) or ornithine (Orn) at two time points during cell growth. Two days after subculturing with Arg, an increase in citrulline (Cit) content was observed, followed by a higher expression of genes related to PA catabolism in the responsive cell line; whereas, in the blocked cell line, we only observed an accumulation of PAs. After 14 d, metabolism was directed towards putrescine accumulation in both cell lines. Exogenous Arg and Orn not only caused a change in cellular contents of PAs, but also altered the abundance of a broader spectrum of amino acids. Specifically, Cit was the predominant amino acid. We also noted changes in the expression of genes related to PA biosynthesis and catabolism. These results indicate that Arg and Orn act as regulators of both biosynthetic and catabolic PA metabolites; however, we suggest that they have distinct roles associated with embryogenic potential of the cells.

Citation

de Oliveira, Leandro Francisco; Navarro, Bruno Viana; Cerruti, Giovanni Victório; Elbl, Paula; Minocha, Rakesh; Minocha, Subhash C.; dos Santos, André Luis Wendt; Floh, Eny Iochevet Segal. 2018. Polyamine- and Amino Acid-Related Metabolism: The Roles of Arginine and Ornithine are Associated with the Embryogenic Potential. Plant and Cell Physiology. 59(5): 1084-1098. https://doi.org/10.1093/pcp/pcy049.

Last updated on: November 14, 2019