Publication Details

Designing specific chloroplast markers for black walnut from a set of universal primers

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Year Published

2003

Publication

In: Van Sambeek, J. W.; Dawson, Jeffery O.; Ponder Jr., Felix; Loewenstein, Edward F.; Fralish, James S., eds. Proceedings of the 13th Central Hardwood Forest Conference; Gen. Tech. Rep. NC-234. St. Paul, MN: U.S. Department of Agriculture, Forest Service, North Central Research Station: 466

Abstract

Chloroplasts are a valuable source of genetic information because their sequence is highly conserved, they undergo little or no recombination, and they are uniparentally inherited. Chloroplast polymorphisms are powerful genetic tools for identifying matrilineal family groups, studying gene flow from seed versus pollen movement, reconstructing phylogeographic colonization, and for elucidating reproductive patterns in natural stands. Here, we sequenced six samples representing the range of black walnut (Juglans nigra L.) using six previously published polymorphic chloroplast markers. These markers were from the atpF intron, 3' to rps2, ORF 77-ORF 82 intergenic, ORF 74b-psbB intergenic, and rp12-rps19 intergenic regions in the chloroplast genome. Data from Weising and Gardner (1999) show short mononucleotide repeats of A, T, and G, both individually and in tandem depending on locus. Preliminary data suggest that in black walnut, polymorphisms in these regions are based on series of A and T repeats, and for some loci indicate considerable differences from all the species previously analyzed. New primers specific for black walnut will be designed based on these polymorphisms. These new primers will be used in a study of genetic diversity across the Central Hardwood region.

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Citation

Victory, Erin; Robichaud, Rodney L.; Woeste, Keith 2003. Designing specific chloroplast markers for black walnut from a set of universal primers. In: Van Sambeek, J. W.; Dawson, Jeffery O.; Ponder Jr., Felix; Loewenstein, Edward F.; Fralish, James S., eds. Proceedings of the 13th Central Hardwood Forest Conference; Gen. Tech. Rep. NC-234. St. Paul, MN: U.S. Department of Agriculture, Forest Service, North Central Research Station: 466

Last updated on: January 30, 2007