Publication Details

Relative quantification of membrane-associated calcium in red spruce mesophyll cells

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Borer, Catherine H.; Schaberg, Paul; Cumming, Jonathan R.

Year Published

1997

Publication

Trees. 12: 21-26.

Abstract

We describe a method for localizing and comparing relative amounts of plasma membrane-associated calcium ions (mCa) in complex tissues and verify the procedure for mesophyll cells of red spruce (Picea rubens Sarg.) needles. This technique incorporates epifluorescence microscopy using the fluorescent probe chlorotetracycline (CTC) with computer image processing and analysis. Using an appropriate standardization for image brightness, the procedure allows relative quantitative comparison of CTC-fluorescence in the plasma membrane-cell wall region that corresponds to relative amounts of mCa. The technique effectively discerned mCa differences among red spruce needle sections exposed to treatments designed to alter mCa levels in vitro.

Citation

Borer, Catherine H.; Schaberg, Paul; Cumming, Jonathan R. 1997. Relative quantification of membrane-associated calcium in red spruce mesophyll cells. Trees. 12: 21-26.

Last updated on: July 30, 2008