Agrobacterium-mediated transformation of black cherry for flowering control and insect resistance
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Plant Cell, Tissue and Organ Culture. 119(1): 107-116.
Black cherry is one of the most valuable hardwood species for cabinetry, furniture, and veneer. The goal of this study was to develop transgenic black cherry plants with reproductive sterility and enhanced insect resistance. Black cherry TERMINAL FLOWER 1 (PsTFL1) was overexpressed under the control of the CaMV 35S promoter in black cherry via Agrobacterium-mediated transformation, as a strategy for gene containment. PsTFL1 is a homolog of Arabidopsis TFL1 which is known to play a key role in regulating flowering time by counteracting with FLOWERING LOCUS T and repressing the transcription of the floral-related genes. The elevated expression level of PsTFL1 was proven to be able to significantly delay flowering and cause abnormal floral structure which led to sterility in Arabidopsis. Therefore, the overexpression of PsTFL1 was expected to induce the similar phenotype in black cherry to achieve reproductive sterility.
KeywordsCyanogenesis, Flowering control, Gene silencing, Genetic transformation, Insect resistance, Prunus serotina
Wang, Ying; Pijut, Paula M. 2014. Agrobacterium-mediated transformation of black cherry for flowering control and insect resistance. Plant Cell, Tissue and Organ Culture. 119(1): 107-116. https://doi.org/10.1007/s11240-014-0517-1.