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Vegetative propagation of butternut (Juglans cinerea) field results

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Year Published

2004

Publication

In: Michler, C.H.; Pijut, P.M.; Van Sambeek, J.W.; Coggeshall, M.V.; Seifert, J.; Woeste, K.; Overton, R.; Ponder, F., Jr., eds. Proceedings of the 6th Walnut Council Research Symposium; Gen. Tech. Rep. NC-243. St. Paul, MN: U.S. Department of Agriculture, Forest Service, North Central Research Station. 37-44

Abstract

Juglans cinerea L. is a hardwood species valued for its wood and edible nuts. Butternut canker disease (Sirococcus clavigignenti-juglandacearum) threatens its survival. Vegetative propagation will be required to produce clones of genotypes selected for resistance to butternut canker disease. In 2000, 10 trees were randomly selected from a 6-year-old butternut plantation located in Rosemount, MN. Hardwood stem cuttings were collected in March, April, and May. Softwood cuttings were collected in June and July. Indole-3-butyric acid-potassium salt (K-IBA) at 0, 29, or 62 mM in water and indole-3-butyric acid (IBA) at 0, 34, or 74 mM in 70% ethanol were tested for root induction on cuttings. The basal end of cuttings were dipped in a treatment solution for 10 to 15 s, potted in a peat:perlite mixture and placed in a mist bed for 5 to 8 weeks. Rooted cuttings were gradually hardened off from the mist bed, allowed to initiate new growth, over-wintered in a controlled cold-storage environment, and then outplanted to the field. Rooting was greatest for hardwood cuttings taken in mid-May (branches flushed out), 22% with 62 mM K-IBA and 28% with 74 mM IBA. Softwood cuttings rooted best when taken in June (current season?s first flush of new growth or softwood growth 40 cm or greater) and treated with 62 mM K-IBA (77%) or 74 mM IBA (88%). One-hundred and seventy three (173) out of 186 rooted softwood cuttings (93%) survived over-wintering and acclimatization to the field. Average heights and stem diameters were after 1 year (28.6 cm, 12.4 mm) or 2 years (92.8 cm, 18.6 mm). When plants were protected from deer browse, rodent damage, and weed controlled, 91% survived in the field.

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Citation

Pijut, Paula M. 2004. Vegetative propagation of butternut (Juglans cinerea) field results. In: Michler, C.H.; Pijut, P.M.; Van Sambeek, J.W.; Coggeshall, M.V.; Seifert, J.; Woeste, K.; Overton, R.; Ponder, F., Jr., eds. Proceedings of the 6th Walnut Council Research Symposium; Gen. Tech. Rep. NC-243. St. Paul, MN: U.S. Department of Agriculture, Forest Service, North Central Research Station. 37-44

Last updated on: August 11, 2006