Establishment and characterization of American elm cell suspension cultures
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Plant Cell, Tissue and Organ Culture. 61: 245-249.
Cell suspension cultures of Dutch elm disease (DED)-tolerant and DED-susceptible American elms clones have been established and characterized as prerequisites for contrasts of cellular responses to pathogen-derived elicitors. Characteristics of cultured elm cell growth were monitored by A700 and media conductivity. Combined cell growth data for all experiments within a genotype showed relatively low variances and between-genotype contrasts during repeated passages showed no significant differences. Subculturing exponentially growing cells at 8-14 day intervals, within readily measured parameters of media conductivity (4.95-4.2 mmhos) and cell concentration (≥1.4 A700), consistently resulted in repeatable profiles of elm cell growth and minimized lag phase. Culture cells were essentially homogeneous after 5 subculture passages and their overall appearance was stable. We conclude that the described procedure resulted in consistent cultures suitable for elicitor treatment experiments.
Keywordscultured cell line, growth rates, tissue culture, Ulmus americana
Eshita, Steven M.; Kamalay, Joseph C.; Gingas, Vicki M.; Yaussy, Daniel A. 2000. Establishment and characterization of American elm cell suspension cultures. Plant Cell, Tissue and Organ Culture. 61: 245-249.