Publication Details
DNA barcodes to identify species and explore diversity in the Adelgidae (Insecta: Hemiptera: Aphidoidea)
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Year Published
2009
Publication
Molecular Ecology Resources. 9 (Suppl. 1): 188-195.
Abstract
The Adelgidae are relatively small, cryptic insects, exhibiting complex life cycles with parthenogenetic reproduction. Due to these characteristics, the taxonomy of the group is problematic. Here, we test the effectiveness of the standard 658-bp barcode fragment from the 5'-end of the mitochondrial cytochrome c oxidase 1 gene (COI) in differentiating among 17 species of Adelgidae, in associating life-cycle stages, and in assessing patterns of geographical variation in selected species. Species of Adelgidae are well-differentiated by DNA barcodes, enabling the identification of different morphological forms, immature stages and individuals on different hosts and at different periods of the life cycle. DNA barcodes have uncovered cryptic diversity within taxa and, in other cases, a lack of sequence divergence in species pairs previously separated by life-cycle characteristics, indicating a need for further taxonomic analysis.
Keywords
Adelgidae COI DNA barcoding life cycles mitochondrial DNA parthenogenesis species identificationCitation
Foottit, R.G.; Maw, H.E.L.; Havill, N.P.; Ahern, R.G.; Montgomery, M.E. 2009. DNA barcodes to identify species and explore diversity in the Adelgidae (Insecta: Hemiptera: Aphidoidea). Molecular Ecology Resources. 9 (Suppl. 1): 188-195. https://doi.org/10.1111/j.1755-0998.2009.02644.x.