Physiological responses of suspension cultures of Catharanthus roseus to aluminum: changes in polyamines and inorganic ions
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Journal of Plant Physiology. 145: 277-284.
The effects of aluminum (Al) treatment on polyamines were studied using suspension cultures of Madagascar periwinkle [Catharanthus roseus (L.) G. Don]. The addition of A1 (0.2, 0.5, 1.0 mM) to the suspension cultures caused a significant increase in putrescine within 24h only in freshly transferred cells. By contrast, Al treatment reduced putrescine content at all other times (days 2-7). Further analysis showed that the largest increase in putrescine occurred at 6h when Al was added at the time of transfer of cells to fresh medium. If Al treatment was delayed to as little as 3h after transfer to fresh medium, the cellular level of putrescine decreased. While spermidine remained unchanged, spermine was significantly higher in the presence of Al in all cases. The inhibitory effect of Al on DNA synthesis also depended upon the time of its addition. Cellular Ca, Mg, and Mn decreased in response to Al given at the time of transfer to fresh media while Fe increased, and Na, K and P remained unchanged. The results indicate a critical physiological change in the response of cells to Al within a short time (about 3h) after transfer to fresh medium.
Keywordsarginine decarboxylase; Catharunthus roseus; DNA synthesis; inorganic ions; polyamines; putrescine; spermidine; spermine
Zhou, Xinhua; Minocha, Rakesh; Minocha, Subhash C. 1995. Physiological responses of suspension cultures of Catharanthus roseus to aluminum: changes in polyamines and inorganic ions. Journal of Plant Physiology. 145: 277-284.