In vitro colony interactions among species of Trichoderma with inference toward biological control.
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Res. Pap. PNW-RP-474. Portland, OR: U.S. Department of Agriculture, Forest Service, Pacific Northwest Research Station. 8 p.
Colony interactions among 15 isolates representing seven species of Trichoderma were evaluated in vitro. Interactions characterized by zones of inhibition, demarcation lines, ridges of conidia, overgrowth, intermingling, anastomosis, and hyphal coiling in self-pairings and intraspecific and interspecific pairings of the seven species were recorded. All types of interactions occurred in interspecific pairings except intermingling in dual culture. Intermingling interactions were most common in self-pairings. Demarcation lines and overgrowth interactions were absent in self-pairings and intraspecific pairings. Microscopic examination of Trichoderma hyphae in the interaction zone between colonies revealed that anastomosis occurred only in self-pairings and intraspecific pairings, whereas circular and lateral coiling occurred in all pairing types. Terminal and intercalary chlamydospores formed in the interaction zones in all pairings. An understanding of the compatibility between species or isolates of Trichoderma will provide information on the use of multiple species of Trichoderma as biological control agents.
Reaves, Jimmy L.; Crawford, Ralph H. 1994. In vitro colony interactions among species of Trichoderma with inference toward biological control. Res. Pap. PNW-RP-474. Portland, OR: U.S. Department of Agriculture, Forest Service, Pacific Northwest Research Station. 8 p. https://doi.org/10.2737/PNW-RP-474.