There is a critical need for information on the mating behavior of Anoplophora glabripennis (ALB) to provide the biological basis for predicting population dynamics, especially as the population size declines due to eradication efforts. In addition, efforts are continuing to try and develop a trapping system for the adults. Understanding the reproductive behaviors of ALB may also aid in identifying its pheromones and developing a trapping system for early detection and confirmation of eradication.
A series of four experiments using three strains (New York, Illinois, and China) was designed to document the following: 1) the reproductive behaviors (both mating and oviposition) of a single pair until natural separation or over 6 hours which ever came first, 2) the number of females a male can successfully mate in its lifetime and how male age affects mating success, 3) the frequency and duration of matings associated with sustained female fertility, and 4) the interactions of all possible combinations (male and female) and sequences of 3 individuals(MMF, MFM, FFM, FMF). Expected Outcomes
Information on dispersal and capacity to spread that is fundamental to development of survey methods and protocols, and to determining establishment potential.
Information on how ALB mating behaviors influence establishment and viability of ALB populations. Clues to possible pheromones that could be used in a trapping system.
The reproductive behaviors of A. glabripennis are typical of diurnally active species of the subfamily Lamiinae. When a male contacted a female with his antennae, he generally would quickly attempt to mount her and mate. If the female was receptive (did not fight the mounting and allowed access to her genital chamber), he would mate with her immediately after mounting and initiate a prolonged pair-bond. Non-receptive females would exhibit one or more of the following behaviors: run away, kick with hind legs, hit with antennae, make quick turns, fall, or fly. In these cases, the male might abandon his attempt and separate or perform a short antennal wagging courtship behavior. During the entire time, the male continuously grasped the female with his front tarsi or both front and middle tarsi. During 6 hour observations, individual copulation events lasted an average of 2.8 minutes and 1-10 copulations occurred per event, followed by a male refractory period averaging 95 and 60 minutes, for the New York and Illinois males respectively. During copulations the female remained stationary, walked or chewed the host for oviposition (the latter occurred more often during later copulation events). Between copulations, the female most often walked or attempted oviposition in the pit she had chewed. The average total time in copula during the 6 hours was 34 minutes and this resulted in an average hatch of 56% of eggs females laid over their lifetime. Oviposition (0-5 eggs per female) lasted an average of 12 and 10 minutes, for the New York and Illinois females respectively on bolts with bark 1-2 mm thick. After a female chewed a pit, she rotated 180°, extended her ovipositor, and used it to find the pit. She then inserted her ovipositor under the bark and used the sclerites at the tip of her abdomen to pry the bark up while lifting her body. After laying an egg, she wiped excretions across the opening using the tip of her abdomen. Females abandoned some pits at various points in the process. Females that had been in pair-bond a long time would often begin to exhibit the non-receptive behaviors again usually just after cleaning their antennae. Male usually responded by wagging their antennae to try and calm the female but if this failed they would dismount if the female did not successfully dislodge them first.
Male beetles 11-104 days of age were able to copulate (> 3 minutes in duration) successfully. Copulation was considered successful if the female laid eggs that hatched. Males generally had one or more unsuccessful copulations prior to the first success. Male fertility (as measured by % egg hatch of females he copulated with) and copulation success peaked for the 3 males tested at 3-5 weeks of age, then slowly declined until copulation stopped at about week 15. When the males reached 12 weeks of age, they were less agile and less able to grasp and hold a female, which in an unconstrained environment could result in a less receptive female escaping. The shortest interval between two successful copulations was 5 minutes (when a second virgin female was presented immediately after a copulation ended). The most females successfully copulated by one male was 27, resulting in a total of 1,366 progeny.
On average, females that had copulated 10 times or more (each > 3 minutes in duration) had a significantly higher percentage of viable eggs than did females with only 1 or 5 copulations, but fecundity was unaffected. The number of copulation events did not significantly affect female longevity. These results suggest that >1 hour of total time in copula (excluding mate guarding time) is needed for maximum sustained fertility, as measured by percentage hatch. In nature, this time requirement for copulation could be satisfied by one or more matings of longer duration (multiple copulations), rather than through a series of short encounters.
During the experiment where 3 beetles (male and female) were put together in different combinations and sequences, our observations suggested that several semiochemicals are likely involved in the mating process. We observed that when males were added after a female was present, one-third of the males walked slowly, palpating the bark as if following a chemical trail directly to the female then mounted the female after his antennae contacted her. We also observed that approximately one-third of the females located the males and made the first contact, but were not mounted until after the male contacted the female with his antennae. If an aggressive male to male encounter occurred first then the percentage of opposite sexes finding each other declined dramatically. When a male mounted a female, he remained with her and fought off both males and females that made contact with them, usually without dismounting. When males dismounted, they were able to relocate the females easily. The loser of a male-to-male encounter flew away 21% of the time and ran away 75% of the time which tended to disperse the males and thus ensured that all females would be found. Twice we observed a male treating another male (that had recently been with a female) like a female, even initiating copulation attempts. These observations suggest that once the two sexes are on the same tree, they will find each other, that a male will stay with a female long enough to ensure she will remain fertile the rest of her life, and that males will disperse themselves to reduce aggressive encounters, thus increasing the likelihood of locating females even when populations are low.
Keena, M.A.; Sánchez, V. 2018. Inter- and Intrasexual Interactions in Anoplophora glabripennis (Coleoptera: Cerambycidae) and the Impact of Different Sex Ratios. Journal of Economic Entomology. 39: 1-. https://doi.org/10.1093/jee/toy207.
Keena, M.A.; Sánchez, V. 2018. Reproductive Behaviors of Anoplophora glabripennis (Coleoptera: Cerambycidae) in the Laboratory. Journal of Economic Entomology. 111(2): 620-628. https://doi.org/10.1093/jee/tox355.
Keena, Melody A.; Sanchez, Vicente. 2007. Reproductive behaviors of Asian longhorned beetle. In: Mastro, Victor; Lance, David; Reardon, Richard; Parra, Gregory, comps. Emerald ash borer and Asian longhorhed beetle research and development review meeting; 2006 October 29-November 2; Cincinnatti, OH. FHTET 2007-04. Morgantown, WV: U.S. Forest Service, Forest Health Technology Enterprise Team: 123-124.
- Melody Keena, USDA Forest Service - Northern Research Station - Research Entomologist
- Vicente Sanchez, USDA Forest Service - Northern Research Station - Research Entomologist
Last Modified: 08/06/2018