Methods to Increase the Availability of Insects for Use in Research
Because not all research on ALB can be conducted in China or at North American sites where this beetle is being eradicated, the ability to mass rear the Asian longhorned beetle is critical to rapid progress on its exclusion, detection, and eradication techniques. Large numbers of beetles in all life stages are needed year-round. Mass rearing of ALB requires not only an artificial diet that supports larval survival and development but also one that can be dispensed quickly and in high volume. Methods to rear all and stockpile some stages were also needed to make mass rearing possible.
Survival and development were compared for larvae from two populations (Chicago, IL, and Queens, NY) on six diets containing varying levels of Fe (69-237 mg/liter) and for three populations (same two plus Hohhot, Inner Mongolia, China) under four larval chill treatments (6, 9, 12, or 16 wk development before chill). Chilling eggs before incubating them for hatch was also evaluated.
A consistently available, high quality supply of ALB for research purposes.
Larval survival and percentage pupation significantly decreased and development time slightly increased with increasing Fe levels in the diet. Larval survival and percentage pupation were highest, adults weighed the most, and development time was shortest when larvae were reared on a pourable modification of the Enaphalodes rufulus (Haldeman) diet. Individuals from the China and Illinois populations were heavier than those from New York. On average, larvae from the Illinois population were ready to pupate sooner than those from New York or China. Some larvae that had not reached their critical weight for pupation before the chill period required a second chill period before initiating pupation. Overall survival increased as the developmental time period prior to chill increased.
Further evaluation of the effects of temperature on development is needed to better understand the triggers for pupation and to predict the timing of various stages.
A summary of diets used for this and other cerambycids is now available and may be useful for developing methods for other species of longhorned beetles.
Keena, MA. 2017. Chapter 7: Laboratory rearing and handling. In: Cerambycidae of the World: Biology and Pest Management. CRC Press/Taylor & Francis Group. Book published Feb. 2017.
Keena, MA. 2005. Pourable artificial diet for rearing Anoplophora glabripennis (Coleoptera: Cerambycidae) and methods to optimize larval survival and synchronize development. Ann., Entomol. Soc. Am. 98(4): 536-547.
- Melody Keena, USDA Forest Service - Northern Research Station - Research Entomologist
Last Modified: 07/14/2017