Northern Research Station
11 Campus Blvd., Suite 200
Newtown Square, PA 19073
(610) 557-4132 TTY/TDD
Title: Agrobacterium-mediated transformation of Fraxinus pennsylvanica hypocotyls and plant regeneration
Author: Du, Ningxia; Pijut, Paula M.
Publication: Plant Cell Reports. 28: 915-923.
Key Words: genetic engineering, green ash, sonication, transgenic, vacuum-infiltration
Abstract: A genetic transformation protocol for green ash (Fraxinus pennsylvanica) hypocotyl explants was developed. Green ash hypocotyls were transformed using Agrobacterium tumefaciens strain EHA105 harboring binary vector pq35GR containing the neomycin phosphotransferase (nptII) and β-glucuronidase (GUS) fusion gene, and an enhanced green fluorescent protein gene. Pre-cultured hypocotyl explants were transformed in the presence of 100 µM acetosyringone using 90 s sonication plus 10 min vacuum-infiltration.
This document is in PDF format. You can obtain a free PDF reader from Adobe.
Forest Service Home | USDA.gov | recreation.gov