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Title: Rapid formation of few polyhedral mutants of Lymantria dispar multinucleocapsid nuclear polyhedrosis virus during serial passage in cell culture
Author: Slavicek, James M.; Hayes-Plazolles, Nancy; Kelly, Mary Ellen.
Publication: Biological Control. 5: 251-261.
Key Words: Lymantria dispar multinucleocapsid nuclear polyhedrosis virus, Lymantria dispar, gypsy moth, cell culture, few polyhedra mutants, biocontrol
Abstract: Four genotypic variants of Lymantria dispar multimucleocapsid nuclear polyhedrosis virus (LdMNPV) were used to investigate the generation of few polyhedra (FP) mutants during viral propagation in the L. dispar 652Y cell line. Titers of budded virus, the percentage of infected cells producting polyhedra, the amount of polyhedra produced per cell, the proportion of virus exhibiting an FP phenotype, virion occlusion characteristics, and changes in genomic DNA digestion profiles were investigated through five serial passages.
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