You are here: NRS Home / Publications & Data / Rapid formation of few polyhedral mutants of Lymantria dispar multinucleocapsid nuclear polyhedrosis virus during serial passage in cell culture

Publication Details

Publication Toolbox
  • Download PDF  (1.1 MB)
  • This publication is available only online.

Rapid formation of few polyhedral mutants of Lymantria dispar multinucleocapsid nuclear polyhedrosis virus during serial passage in cell culture

Year Published

1995

Source

Biological Control. 5: 251-261.

Abstract

Four genotypic variants of Lymantria dispar multimucleocapsid nuclear polyhedrosis virus (LdMNPV) were used to investigate the generation of few polyhedra (FP) mutants during viral propagation in the L. dispar 652Y cell line. Titers of budded virus, the percentage of infected cells producting polyhedra, the amount of polyhedra produced per cell, the proportion of virus exhibiting an FP phenotype, virion occlusion characteristics, and changes in genomic DNA digestion profiles were investigated through five serial passages.

Keywords

Citation

Slavicek, James M.; Hayes-Plazolles, Nancy; Kelly, Mary Ellen. 1995. Rapid formation of few polyhedral mutants of Lymantria dispar multinucleocapsid nuclear polyhedrosis virus during serial passage in cell culture. Biological Control. 5: 251-261.
Last updated on: August 23, 2010

NRS at a Glance