Rakesh Minocha
Title: Plant Physiologist / Biochemist
Unit: Biological and Environmental Influences on Forest Health and Productivity
Address: Northern Research Station
271 Mast Road
Durham, NH 03824
Phone: 603-868-7622
E-mail: Contact Rakesh Minocha
Jump to Publications
Education
- Ph.D. Biochemistry, University of New Hampshire, Durham, NH, 1985
- M.S. Zoology, University of New Hampshire, Durham, NH, 1978
- M.Sc. (Honors), Zoology, Panjab University, Chandigarh, India, 1976
- B.Sc. (Honors), Zoology, Panjab University, Chandigarh, India, 1975
Civic & Professional Affiliations
American Society of Plant Biologists, Ecological Society of America, International Society of Environmental Bioindicators
Current Research
Current research:
- My research is focused on the physiological response of trees to injury, infection, and environmental change. Sources of injury may be obvious such as those from fire, storms, pests, pathogens and human activity. Significant, yet less obvious change may come from perturbations in soil chemistry due to acid rain.
Current research examines:
- As a part of various interdisciplinary research teams, I examine wood and foliar chemistry (including inorganic cations, polyamines, amino acids, soluble proteins, and chlorophyll) at various sites in the Northeastern USA, Europe and Asia to determine the correlation between these parameters and other data on root, soil, and soil solution chemistry collected by our cooperators and collaborators. So far, putrescine, a polyamine, and arginine, an amino acid have shown the potential to be used as early indicators of physiological stress under field conditions.
- The applicability and limitations of dendrochemistry to provide markers of environmental change.
Why is This Important
Stress from mechanical injury, subsequent infection, and environmental change are facts of life for wild, rural, and urban trees. This stress often impacts the diverse goals of forest management, wildlife conservation, high-quality wood products, or the desire for safe and healthy trees in our communities. Maximizing the benefits of trees for forests and communities requires understanding how these goals are linked to tree biology and early detection of tree response to environmental change.
Future Research
- Keep working on the current research focus and add new indicators to our currently existing list that can predict changes in forest productivity.
- Using molecular techniques, study the effects of environmental changes such as excess of nitrogen on microbial population intensity and/or diversity and ecosystem functions.
- Develop new techniques or optimize existing techniques that can help us analyze tree samples for additional physiological indicators.
- Write articles in trade journals or magazines of general public interest to transfer this technology on to environmental scientists and scientists belonging to other disciplines. This transfer will help the public understand the value of our research.
Featured Publications
Additional
Online Publications
- Sridevi, Ganapathi; Minocha, Rakesh; Turlapati, Swathi A.; Goldfarb, Katherine C.; Brodie, Eoin L.; Tisa, Louis S.; Minocha, Subhash C. 2012. Soil bacterial communities of a calcium-supplemented and a reference watershed at the Hubbard Brook Experimental Forest (HBEF), New Hampshire, USA. FEMS Microbiology Ecology. 79: 728-740.
- Schaberg, Paul G.; Minocha, Rakesh; Long, Stephanie; Halman, Joshua M.; Hawley, Gary J.; Eagar, Christopher. 2011. Calcium addition at the Hubbard Brook Experimental Forest increases the capacity for stress tolerance and carbon capture in red spruce (Picea rubens) trees during the cold season. Trees. 25: 1053-1061.
- Minocha, Rakesh; Long, Stephanie; Minocha, Subhash; Marquardt, Paula; Nelson, Neil; Kubiske, Mark. 2010. Effects of long-term (10 years) exposure to elevated CO2 and O3 on trembling Aspen carbon and nitrogen metabolism at the aspen FACE (Free-Air Carbon Dioxide Enrichment) study site. In: Joint annual meetings of the American Society of Plant Biologists and the Canadian Society of Plant Physiologists; 2010 July 31- Aug 4; Montreal, Canada: Plant Biology 377. Poster Abstract.
- Minocha, Rakesh; Long, Stephanie; Minocha, Subhash; Marquardt, Paula; Nelson, Neil; Kubiske, Mark. 2010. Effects of long-term (10 years) exposure to elevated CO2 and O3 on trembling Aspen carbon and nitrogen metabolism at the aspen FACE (Free-Air Carbon Dioxide Enrichment) study site. In: 95th Ecological Society of America annual meeting; 2010 August 1-6; Pittsburg, PA. Abstract.
- Mohapatra, Sridev; Minocha, Rakesh; Long, Stephanie; Minocha, Subhash C. 2010. Transgenic manipulation of a single polyamine in poplar cells affects the accumulation of all amino acids. Amino Acids. 38: 1117-1129.
- Smith, Kevin T.; Shortle, Walter C.; Connolly, Jon H.; Minocha, Rakesh; Jellison, Jody. 2009. Calcium fertilization increases the concentration of calcium in sapwood and calcium oxalate in foliage of red spruce. Environmental and Experimental Botany. 67: 277-283.
- Minocha, Rakesh; Martinez, Gabriela; Lyons, Benjamin; Long, Stephanie. 2009. Development of a standardized methodology for quantifying total chlorophyll and carotenoids from foliage of hardwood and conifer tree species. Canadian Journal of Forest Research 39:849-861
- Mohapatra, Sridev; Minocha, Rakesh; Long, Stephanie. 2009. Putrescine overproduction negatively impacts the oxidative state of poplar cells in culture. Plant Physiology and Biochemistry 47: 262-271.
- Shortle, Walter C.; Murdoch, Peter S.; Smith, Kevin T.; Minocha, Rakesh; Lawrence, Gregory B. 2008. 6.0 Monitoring recovery from calcium depletion and nitrogen saturation. In: Murdoch, Peter S.; Jenkins, Jennifer C.; Birdsey, Richard A. The Delaware River Basin Collaborative Environmental Monitoring and Research Initiative. Gen. Tech. Rep. NRS-25. Newtown Square, PA: U.S. Department of Agriculture, Forest Service, Northern Research Station: 52-62.
- Serapiglia, Michelle J.; Minocha, Rakesh; Minocha, Subhash C. 2008. Changes in polyamines, inorganic ions and glutamine synthetase activity in response to nitrogen availability and form in red spruce (Picea rubens). Tree Physiology. 28: 1793-1803.
- Minocha, Rakesh; Thangavel, P.; Dhankher, Om Parkash; Long, Stephanie. 2008. Separation and quantification of monothiols and phytochelatins from a wide variety of cell cultures and tissues of trees and other plants using high performance liquid chromatography. Journal of Chromatography A. 1207: 72-83.
- Thangavel, P.; Long, Stephanie; Minocha, Rakesh. 2007. Changes in phytochelatins and their biosynthetic intermediates in red spruce (Picea rubens Sarg.) cell suspension cultures under cadmium and zinc stress. Plant Cell Tissue Organ Culture. 88: 201-216.
- Page, Andrew F.; Mohapatra, Sridev; Minocha, Rakesh; Minocha, Subhash C. 2007. The effects of genetic manipulation of putrescine biosynthesis on transcription and activities of the other polyamine biosynthetic enzymes. Physiologia Plantarum. 129: 707-724.
- Minocha, Rakesh; Minocha, Subhash C. 2005. Effects of soil pH and aluminum on plant respiration. In; Lambers, H.; Ribas-Carbo, M., eds. Plant respiration: from cell to ecosystem. Advances in photosynthesis and respiration, vol 18. Dordrecht, The Netherlands: Springer: 159-176.
- Dujesiefken, Dirk; Liese, Walter; Shortle, Walter; Minocha, Rakesh. 2005. Response of beech and oaks to wounds made at different times of the year. European Journal of Forest Research. 124: 113-117.
- Bauer, G.A.; Bazzaz, F.A.; Minocha, R.; Long, S.; Magill, A.; Aber, J.; Berntson, G.M. 2004. Effects of chronic N additions on tissue chemistry, photosynthetic capacity, and carbon sequestration potential of a red pine (Pinus resinosa Ait.) stand in the NE United States. Forest Ecology and Management. 196: 173?186.
- Minocha, Rakesh; Minocha, Subhash C.; Long, Stephanie. 2004. Polyamines and their biosynthetic enzymes during somatic embryo development in red spruce (Picea rubens Sarg.). In Vitro Cell. Dev. Biol.-Plant 40:572-580
- Minocha, S.C.; Bhatnagar, P.; Minocha, R. 2003. Genetic manipulation of polyamine metabolism in poplar. In: Espinel, S.; Barredo, Y.; Ritter, E., eds. Sustainable forestry, wood products, and biotechnology. Victoria-Gasteiz, Spain: DFA-AFA Press: 179-194.
- Wargo, Philip M.; Minocha, Rakesh; Wong, Betty L.; Long, Robert P.; Horsley, Stephen B.; Hall, Thomas J. 2003. Measuring stress and recovery in limed sugar maple on the Allegheny Plateau of north-central Pennsylvania. In: Fosbroke, Sandra L.C.; Gottschalk, Kurt W., eds. Proceedings, U.S. Department of Agriculture interagency research forum on gypsy moth and other invasive species 2002; 2002 January 15-18; Annapolis, MD. Gen. Tech. Rep. NE-300. Newtown Square, PA: U.S. Department of Agriculture, Forest Service, Northeastern Research Station: 101.
- Minocha, R.; Minocha, S.C.; Long, S. 2003. Monitoring environmental stress in forest trees using biochemical and physiological markers. In: Espinel, S.; Barredo, Y.; Ritter, E., eds. Sustainable forestry, wood products, and biotechnology. Victoria-Gasteiz, Spain: DFA-AFA Press: 109-116.
- Quan, Yi; Minocha, Rakesh; Minocha, Subhash C. 2002. Genetic manipulation of polyamine metabolism in poplar II: effects on ethylene biosynthesis. Plant Physiology and Biochemistry. 40: 929-937.
- Bhatnagar, Pratiksha; Minocha, Rakesh; Minocha, Subhash C. 2002. Genetic manipulation of the metabolism of polyamines in poplar cells. The regulation of putrescine catabolism. Plant Physiology. 128: 1455-1469.
- Wargo, Philip M.; Minocha, Rakesh; Wong, Betty; Long, Robert P.; Horsley, Stephen B.; Hall, Thomas J. 2002. Measuring changes in stress and vitality indicators in limed sugar maple on the Allegheny Plateau in north-central Pennsylvania. Canadian Journal of Forest Research. 32: 629-641.
- Minocha, R.; Minocha, S.C.; Long, S. 2002. Monitoring environmental stress in forest trees using biochemical and physiological markers. In: International Congress: Sustainable forestry wood products & biotechnology (Biofor-02); 2002 November 11-14; Victoria-Gasteiz, Spain. 109-116.
- Minocha, Rakesh; McQuattie, Carolyn; Fagerberg, Wayne; Long, Stephanie; Noh, Eun Woon. 2001. Effects of aluminum in red spruce (Picea rubens) cell cultures: Cell growth and viability, mitochondrial activity, ultrastructure and potential sites of intracellular aluminum accumulation. Physiologia Plantarum. 113: 486-498.
- Minocha, Rakesh; Long, Stephanie; Magill, Alison H.; Aber, John; McDowell, William H. 2000. Foliar free polyamine and inorganic ion content in relation to soil and soil solution chemistry in two fertilized forest stands at the Harvard Forest, Massachusetts. Plant and Soil. 222: 119-137.
- Minocha, R.; Jain, S.M. 2000. Tissue culture of woody plants and its relevance to molecular biology. In: Jain, S.M.; Minocha, S.C., eds. Molecular biology of woody plants. The Netherlands: Kluwer Academic Publishers: 315-340.
- Shortle, Walter C.; Smith, Kevin T.; Minocha, Rakesh; Minocha, Subhash; Wargo, Philip M.; Vogt, Kristina A. 2000. Tree health and physiology in a changing environment. In: Mickler, Robert A.; Birdsey, Richard A.; Hom, John, eds. Responses of northern U.S. forests to environmental change. Ecological studies 139. New York: Springer-Verlag: 229-274.
- Minocha, Rakesh; Long, Stephanie; Maki, Hisae; Minocha, Subhash C. 1999. Assays for the activities of polyamine biosynthetic enzymes using intact tissues. Plant Physiology and Biochemistry. 37: 597-603.
- Minocha, S.C.; Minocha, R. 1999. Genetic transformation in conifers. In: Jain, S.M.; Gupta, P.K., eds. Somatic embryogenesis in woody plants. Vol. 5. The Netherlands: Kluwer Academic Publishers: 291-312.
- Minocha, Rakesh. 1999. Markers of environmental stress in forest trees. In: Horsley, Stephen B.; Long, Robert P., eds. Sugar maple ecology and health: proceedings of an international symposium; 1998 June 2-4; Warren, PA. Gen. Tech. Rep. NE-261. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Research Station: 106.
- Minocha, Rakesh; Smith, Dale R.; Reeves, Cathie; Steele, Kevin D.; Minocha, Subhash C. 1999. Polyamine levels during the development of zygotic and somatic embryos of Pinus radiata. Physiologia Plantarum. 105: 155-164.
- Shortle, Walter C.; Minocha, Rakesh. 1999. Taxol: a review. In: Raychaudhuri, S.P.; Maramorosch, K., eds. Biotechnology and plant protection in forestry science. [Place Publication unknown]: Science Publishers: 1-11.
- Shortle, Walter C.; Smith, Kevin T.; Minocha, Rakesh; Minocha, Subhash C.; Wargo, Philip M.; Vogt, Kristiina A. 1999. Tree health and physiology in a changing environment. In: Mickler, Robert A.; Birdsey, Richard A.; Hom, John, eds. Ecological studies: responses of northern U.S. forests to environmental change. Vol. 139. Springer Verlag: 229-274.
- Shortle, Walter C.; Smith, Kevin T.; Minocha, Rakesh; Lawrence, Gregory B.; David, Mark B. 1997. Acidic deposition, cation mobilization, and biochemical indicators of stress in healthy red spruce. Journal of Environmental Quality. 26: 871-876.
- Minocha, Rakesh; Shortle, Walter C.; Lawrence, Gregory B.; David, Mark B.; Minocha, Subhash C. 1997. Relationships among foliar chemistry, foliar polyamines, and soil chemistry in red spruce trees growing across the northeastern United States. Plant and Soil. 191: 109-122.
- Smith, Kevin T.; Shortle, Walter C.; Minocha, Rakesh; Alexeyev, Vladislav A. 1996. Dynamics of calcium concentration in stemwood of red spruce and Siberian fir. In: Hom, John; Birdsey, Richard; O'Brian, Kelly, eds. Proceedings 1995 meeting of the northern global change program; Gen. Tech. Rep. NE-214. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station: 230-238.
- Minocha, Rakesh; Shortle, Walter C.; Coughin, Daniel J. Jr.; Minocha, Subhash C. 1996. Effects of aluminum on growth, polyamine metabolism, and inorganic ions in suspension cultures of red spruce (Picea rubens). Canadian Journal of Forest Research. 26: 550-559.
- Minocha, Rakesh; Shortle, Walter C.; Lawrence, Gregory B.; Mark, B. David; Minocha, Subhash C. 1996. Putrescine: a marker of stress in red spruce trees. In: Hom, John; Birdsey, Richard; O'Brian, Kelly., eds. Proceedings, 1995 meeting of the Northern Global Change Program; 1995 March 14-16; Pittsburgh, PA. Gen. Tech. Rep. NE-214. Radnor, PA: U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station. Radnor, PA. U.S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station: 119-130.
- Minocha, Rakesk; Shortle, Walter C.; Lawrence, Gregory B.; David, Mark B.; Minocha, Subhash C. 1996. Putrescine: a marker of stress in red spruce trees. In: Hom, John; Birdsey, Richard; O'Brian, Kelly, eds. Proceedings 1995 meeting of the northern global change program; Gen. Tech. Rep. NE-214. Radnor, PA: U. S. Department of Agriculture, Forest Service, Northeastern Forest Experiment Station: 119-130.
- Minocha, Subhash C.; Minocha, Rakesh. 1995. Historical aspects of somatic embryogenesis in woody plants. In: Jain, S.; Gupta, P.; Newton, R., eds. Somatic embryogenesis in woody plants. Vol. I. Dordrecht, The Netherlands: Kluwer Academic Publishers: 9-22.
- Zhou, Xinhua; Minocha, Rakesh; Minocha, Subhash C. 1995. Physiological responses of suspension cultures of Catharanthus roseus to aluminum: changes in polyamines and inorganic ions. Journal of Plant Physiology. 145: 277-284.
- Minocha, S.C.; Minocha, R. 1995. Role of polyamines in somatic embryogenesis. In: Bajaj, Yps, ed. Somatic embryogenesis and synthetic seed, Vol. 30. London, UK: Springer-Verlag Publishers: 53-70.
- Shortle, W.C.; Smith, K.T.; Minocha, R.; Alexeyev, V.A. 1995. Similar patterns of change in stemwood calcium concentration in red spruce and Siberian fir. Journal of Biogeography. 22: 467-473.
- Minocha, Rakesh; Minocha, Subhash C.; Simola, Liisa Kaarina. 1995. Somatic embryogenesis and polyamines in woody plants. In: Jain, S.; Gupta, P.; Newton, R., eds. Somatic embryogenesis in woody plants. Vol. I. Dordrecht, The Netherlands: Kluwer Academic Publishers: 337-359.
- Minocha, Rakesh; Shortle, Walter C.; Long, Stephanie L.; Minocha, Subhash C. 1994. A rapid and reliable procedure for extraction of cellular polyamines and inorganic ions from plant tissues. Journal of Plant Growth Regulation. 13: 187-193.
- Minocha, Rakesh; Shortle, Walter C. 1994. Polyamines and inorganic ions extracted from woody tissues by freeze-thawing. In: Vozzo, J.A., comp. Research and applications of chemical sciences in forestry, proceedings of the 4th Southern Station Chemical Society; 1994 February 1-2; Starkville, MS. Gen. Tech. Rep. SO-104. New Orleans, LA: U.S. Department of Agriculture, Forest Service, Southern Forest Experiment Station: 28-37.
- Minocha, Rakesh; Shortle, Walter C.; Minocha, Subhash C. 1994. Somatic embryogenesis in Picea abies (Norway spruce) and Picea Rubens (red spruce): growth rates and polyamines. In: Proceedings of papers presented at the second international symposium on applications of biotechnology to tree culture, protection, and utilization; 1994 October 2-6; Bloomington, MN. Gen. Tech. Rep. NC-175, St. Paul, MN: U.S. Department of Agriculture, Forest Service, North Central Forest Experiment Station: 20.
- Minocha, Rakesh; Shortle, Walter C. 1993. Fast, safe, and reliable methods for extraction of major inorganic cations from small quantities of woody plant tissues. Canadian Journal of Forest Research. 23: 1645-1654.
- Minocha, Rakesh; Kvaalen, Haarald; Minocha, Subhash C.; Long, Stephanie. 1993. Polyamines in embryogenic cultures of Norway spruce (Picea abies) and red spruce (Picea rubens). Tree Physiology. 13: 365-377.
- Minocha, Rakesh; Minocha, Subhash C.; Long, Stephanie L.; Shortle, Walter C. 1992. Effects of aluminum on DNA synthesis, cellular polyamines, polyamine biosynthetic enzymes and inorganic ions in cell suspension cultures of a woody plant, Catharanthus roseus. Physiologia Plantarum. 85: 417-424.
- Minocha, S.C.; Minocha, R.; Komamine, A. 1991. Effects of polyamine biosynthesis inhibitors on S-adenosylmethionine synthetase and S-adenosylmethionine decarboxylase activities in carrot cell cultures. Plant Physiology and Biochemistry. 29: 231-237.
- Minocha, R.; Minocha, S.C.; Komamine, A.; Shortle, W.C. 1991. Regulation of DNA synthesis and cell division by polyamines in Catharanthus roseus suspension cultures. Plant Cell Reports. 10: 126-130.
- Shortle, Walter C.; Minocha, Rakesh. 1990. Applications of ion chromatography to study pollution effects on forest trees. Advanced Ion Chromatography. 2: 299-309.
- Minocha, Subhash C.; Minocha, Rakesh; Robie, Cheryl A. 1990. High-performance liquid chromatographic method for the determination of dansyl-polyamines. Journal of Chromatography. 511: 177-183.
Other Publications by Rakesh Minocha *
- Juice, Stephanie M.; Fahey, Timothy J.; Siccama, Thomas G.; Driscoll, Charles T.; Denny, Ellen G.; Eagar, Christopher; Cleavitt, Natalie L.; Minocha, Rakesh; Richardson, Andrew D. 2006 Response of sugar maple to calcium addition to northern hardwood forest. Ecology. 87(5): 1267-1280.
- Zhang, Qingyuan; Xiao, Xiangming; Braswell, Bobby; Linder, Ernst; Ollinger, Scott; Smith, Marie-Louise; Jenkins, Julian P.; Baret, Fred; Richardson, Andrew D.; Moore,Berrien, III; Minocha, Rakesh. 2006. Characterization of seasonal variation of forest canopy in a temperate deciduous broadleaf forest, using daily modis data. Remote Sensing of Environment. 105: 189-203.
*Due to policy or copyright restrictions, we are unable to provide full-text versions of these publications. Please check with your local library or Contact Rakesh Minocha to inquire about reprints.
Last Modified:
02/15/2012